Drug Discovery and Design Lab 2
Autor: msg123 • January 26, 2018 • Exam • 1,182 Words (5 Pages) • 554 Views
Page 1 of 5
Eznyme Kinetics Assignment
Part 1)
[p-NPP] (mM) | ΔAbs | Velocity (nmol/min) | 1/[p-Npp] | 1/velocity | [p-NPP]/velocity | velocity/[p-NPP] |
0 | 0.02 | 1.4 |
| 0.725 | 0.0000 |
|
0.1 | 0.15 | 10.4 | 10.00 | 0.096 | 0.0096 | 103.793 |
0.2 | 0.28 | 19.5 | 5.00 | 0.051 | 0.0102 | 97.690 |
0.4 | 0.35 | 24.0 | 2.50 | 0.042 | 0.0167 | 60.017 |
0.6 | 0.40 | 27.8 | 1.67 | 0.036 | 0.0216 | 46.253 |
0.8 | 0.46 | 32.0 | 1.25 | 0.031 | 0.0250 | 39.940 |
1 | 0.41 | 28.5 | 1.00 | 0.035 | 0.0351 | 28.476 |
1.2 | 0.46 | 31.8 | 0.83 | 0.031 | 0.0377 | 26.517 |
2 | 0.48 | 33.2 | 0.50 | 0.030 | 0.0603 | 16.583 |
Table 1: Conversion table from results[pic 1][pic 2]
Vmax: 35 nmol/min
Km: 0.35 mM Vmax: 37.549 nmol/min
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