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Isolating Cheek Cell Dna

Autor:   •  September 30, 2015  •  Lab Report  •  283 Words (2 Pages)  •  1,824 Views

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Name: Erica Smallwood

Cheek Cell DNA-Lab Report Sheet

Exercise 1 – Isolating Cheek Cell DNA

Analysis of Results

Appearance of Solution

What is happening to the cells/DNA?

After lysis buffer

The solution had white material floating and is somewhat cloudy

DNA extraction, cells outer layers are breaking open

After alcohol added

Top layer clear, bottom layer cloudy with white material floating

DNA separated from other molecules in the cell extract and DNA is precipitated.

After alcohol mixed

Cloudy, saw several stringy white pieces floating around that seemed bouncy in the middle of the tube

DNA precipitated and aggregates until seen by naked eye, other molecules in the cell extract remain dissolved and not visible.

Questions:

1. Why is cell lysis the first step in isolating DNA from your cells?

Cell lysis is the first step in isolating DNA from your cells because the cells have to be broken down into smaller components to release the DNA.

2. Lysis buffer typically contains detergent.  Why would this help to lyse the cells?

Detergent helps lyse the cells by dissolving and breaking down oils, since most cells are oil-based.

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