Tommy

Scheme of Activities

 Minor Project - 2015-16 (VI Semester)

1. Microorganism

Microorganisms (Bacteria) were isolated from the soil around Hubballi city and maintained on a suitable enrichment medium  (defined separately for amylase and protease producers) and sub-cultured at regular intervals.

Composition of enrichment media (for Protease producers) (g/L)(pH 7.5)

 Casein         5.0

Yeast extract         2.5

 Glucose         1.0

Agar Agar        20

Note- Initially dissolve casein in water at pH 7.5 and then add the remaining components)

Composition of enrichment media (for Amylase producers) (g/L)(pH 7.0)

 Starch         20

Yeast extract         2.5

 Glucose         1.0

Agar Agar        20

Note- Initially dissolve starch in water and then add the remaining components)

The strains were maintained by sub-culturing  at regular interval in the Department.

2. Inoculum Preparation

Microorganisms used in the project were identified as __________. A loop full of culture from slant was transferred aseptically to 100 ml conical flask containing 50 ml of sterilized inoculum medium (sterilized at 121°C for 15 minutes) in laminar air flow. The flasks were incubated at 37 °C for 24 h. and used as inoculum for fermentation process.

3. Submerged fermentation

Prepare 100 ml seed culture medium of the above composition in 250 ml conical flasks. Adjust the pH to 7.0 with 0.1 N NaOH. A mixture containing Glucose and CaCl2 is to be taken in one flask while the remaining medium components are taken in another flask. The above two flasks are sterilized separately and reconstituted in laminar hood after cooling. To the prepared media, add    3 % inoculum under aseptic conditions in laminar flow. After inoculation keep fermentation media at room temperature in rotary flask shaker (120 rpm) for defined incubation period (2 days).