Isolation and Isomerization of Lycopene from Tomato Paste

Title: Isolation and Isomerization of lycopene from tomato paste

Purpose: The purpose of this lab is to extract the caroteriod pigment from connal tomato paste and separate them by column chromatography to obtain a solution that contains lycopene. The usage of ultraviolet visible spectrometry will be used to verify the molecule of the lycopene.

Chemical Information:

Acetone

Molecular Weight: 58.08 g/mol

Chemical Formula: (CH3)2CO

Boiling Point: 56.2C

Melting Point: -95.35C

Density: 0.79 g/mL

Low-boiling Petroleum Ether

Molecular Weight: N/A

Chemical Formula: NA

Boiling Point: 90 C

Melting Point: < -73C

Density: 0.69 g/mol

Sodium Chloride

Molecular Weight: 58.44 g/mol

Chemical Formula: NaCl

Boiling Point: 1413C

Melting Point: 801C

Density: 2.165 g/mL

pH: 6.7 - 7.3 (aqueous solution)

Sodium sulfate, white

Molecular Weight: 142.04 g/mol

Chemical Formula: Na2 SO4

Boiling Point: 1100C

Melting Point: 888C

Density: 2.671 g/mol

Potassium carbonate

Molecular Weight: 138.21 g/mol

Chemical Formula: K2CO3

Boiling Point: Decomposes

Melting Point: 891C

Density: 2.29 g/mL

pH: 11.6 Aqueous solution

Hexane

Molecular Weight: 86.18

Chemical Formula: CH3 CH2)4CH3 n-hexane

Boiling Point: 68C (

Melting Point: -95C

pH: NA

Signs of Exposure: These chemicals are extremely flammable liquid and vapors. They can cause flash fire. Harmful if swallowed or inhaled. It can cause irritation to skin, eye and respiratory tract. If contact, rinse with copious water, and if irritation still see doctor.